Aqua / Biotechnology /
/ Yeast Plus
Profitable aquaculture depends on good water quality.
Physiologically, aquatic animals respond more intensely
to their environment than do terrestrial animals. The
stress of poor water can lead to disease and poor
nutrition and retarded growth of cultivated aquatic
animals. In Aquaculture the importance of Dissolved
Oxygen is well known and it is one of the primary
limiting factors of water quality parameters for success
of aqua farming practices. The significance of Dissolved
Oxygen to improve the ability of microorganisms to
transform aquaculture pond contaminants to final
products which are harmless to shrimp, fish, and
environment has been a subject of laboratory
investigations since the 90-ies. Now the oxidative
microbiological treatment of contaminated soils and
water is the technique of treatment of remediating
contaminated aquaculture ponds. ZenLife is the
combination of Oxygen releasing agent and multi strain
probiotics formulation to address the water quality
issues to enhance livability and sustainability by
increasing Dissolved Oxygen levels in shrimp and fish
Intensification of shrimp
(P. vannamei, P. monodon) and fish (Carps, Pangassius,
Tilapia, Seabass etc.) aquaculture practices resulted in
a total departure from nature and resulted in increased
disease incidence and other problems relating to
productivity and environmental quality. Currently, our
aim is to bring nature back into aquaculture systems by
employing the principles of natural ecosystem to enhance
productivity and make it a sustainable enterprise.
Disease management, productivity and environment
protection are the three key factors for successful and
In aquaculture systems, mostly Dissolved Oxygen is
typically one of the major limiting factors and it
causes sudden mortalities when the level of Dissolved
Oxygen goes down beyond < 1 ppm. Dissolved Oxygen has
essential role in all metabolic activities of shrimp
including feed digestion, moulting and growth. At the
same time Dissolved Oxygen limits the beneficial
probiotics which are capable of aerobically biodegrading
contaminants in the process of Bioremediation in
aquaculture ponds. Without adequate Dissolved Oxygen,
contaminated degradation will either cease or will
proceed by a much slower anaerobic rate.
Oxygen Depletion in Aquaculture Ponds:
Oxygen depletion refers to low levels of Dissolved
Oxygen and may result in shrimp or fish mortality. A
concentration of >5 mg/L Dissolved Oxygen is recommended
for optimum health and growth of shrimp and fish.
Sensitivity to low levels of Dissolved Oxygen is species
specific; however, most species of aquaculture are
distressed when Dissolved Oxygen falls to 2-4 mg/L.
Mortality usually occurs at concentrations less than 2
mg/L. The number of shrimp or fish that die during an
oxygen depletion event is determined by how low the
Dissolved Oxygen gets and how long it stays down.
Effect of Oxygen Depletion in
Oxygen depletion occurs when oxygen consumption exceeds
oxygen production. Increases in oxygen consumption can
be caused by an over-abundance of algae, "turnover" of a
pond water (Thermal Stratification), increased organic
waste entering the pond (i.e., waste feed or excess
feed, faecal matter), death and decay of organic matter
(i.e., algae, shrimp or fish die-offs), or by improperly
dried pond having with sludge that remove oxygen
directly from the water column.
Improved Dissolved Oxygen levels including Integrated
Multi-trophic Aquaculture are now gaining interest for
increasing in situ removal of organic waste in
aquaculture systems. The use of a combination of Oxygen
releaser and multi strain probiotics including
nitrifying bacterial formulation will address the water
quality issues by controlling the ammonia and organic
sludge within the pond system. In view of this Neospark
brings an innovative and unique product ZenLife for
enhanced levels of Dissolved Oxygen and thereby improved
Oxidative Bioremediation of organic waste of aquaculture
Oxidative Bioremediation is one of the Advanced
and balanced Biotechnological tools and is the
application of a biological treatment, mainly beneficial
microbes, to clean-up hazardous contaminants in soil and
surface or subsurface waters by the use of Oxygen in
aquaculture ponds. In Oxidative Bioremediation
microorganisms uses Dissolved Oxygen, which is readily
available in ZenLife, to degrade organic contaminants in
soil, sludge, water and solids. The beneficial
probiotics of ZenLife feed on the contamination,
deriving nutrition for growth and for reproduction with
the help of Dissolved Oxygen. The beneficial probiotics
of ZenLife will survive in the presence of proper
dissolved oxygen levels and consume the waste organic
matter source until the unwanted pollutant is gets
general a successful bioremediation requires:
- Availability of
specific nutrients (N, P, K etc.) to support growth of
these bacterial species
- Abundance of
electron acceptors (i.e. Dissolved Oxygen and
secondary electron acceptors)
- Contact with the
dissolved and adsorbed contaminants
If any one of these
requirements is not fulfilled, bioremediation will be
inhibited, either partially or completely.
bioremediation of ZenLife is more than just
simply adding oxygen and a certain species of bacteria.
Oxidative (Aerobic) bioremediation involves microbial
reactions that require oxygen to go forward. The
bacteria use a carbon substrate as the electron donor
and oxygen as the electron acceptor. Whereas anaerobic
bioremediation involves microbial reactions occurring in
the absence of oxygen and encompasses many processes,
including fermentation, methanogenesis, reductive
dechlorination and sulfate- and nitrate reducing
conditions. Depending on the contaminant, a subset of
these activities may be cultivated. In anaerobic
metabolism, nitrate, sulfate, carbon dioxide, oxidized
materials or organic compounds may replace oxygen as the
electron acceptor and thereby the end products are toxic
to shrimp and fish (EPA 2006).
ZenLife is a unique
formulas for the oxidative bioremediation of ponds, is
standardized at a total count of 5 billion CFU /gm,
which will multiply in a pond to 150 billion CFU/gm in
ZenLife is a state-of-the-art oxygen source for
stimulating in situ oxidative bioremediation in
aquaculture ponds. ZenLife enables aerobic
beneficial probiotics to significantly accelerate rates
of bioremediation over longer periods of time.
The oxygen and probiotics of ZenLife assist in
degradation of excess food, feces, dead algae, reduction
of turbidity thereby improved water and soil quality for
better survival and growth of shrimp and fish in
The use of ZenLife
limits the presence of pathogenic microorganisms.
ZenLife is ideal for use in all types of aquaculture
ponds and garden aquaria to combat the most common
problems associated with high nutrient levels.
ZenLife for ammonia reduction:
In aquaculture ponds, ZenLife is added life for
ammonia reduction through nitrifying bacteria, as the
ZenLife is designed to control ammonia and nitrite.
Nitrification is the biological process in which ammonia
is oxidized to nitrite by Nitrosomonas sp. and nitrite
oxidized to nitrate by Nitrobacter sp.
- Improves Dissolved
Oxygen levels in pond water column.
- Immediate relief to
shrimp and fish during low Dissolved Oxygen levels.
- Eliminates ammonia,
nitrite and hydrogen sulfide through improved
- Reduces BOD and COD
levels and eliminates anaerobic condition.
- Protects from stress
and improves survival rates.
- Controls stock
losses due to low oxygen and high ammonia levels.
- Reduce sludge
buildup at pond bottom.
Shrimp and Fish Pond: 2-3 kg per acre or as advised
by Aquaculture Consultant.
Application procedure: Broadcast uniformly
throughout the pond.
Composition: Concentrated Oxygen releasing
formulation in granular form containing Bacillus sp.,
Nitrosomonas sp., Nitrobacter sp., Rhodobacter sp.,
Presentation: 5 kg
For Servicing Aquaculture Industry (Including Academic
and Research Institutions, Universities etc.)
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Using Slow Oxygen Release
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