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Profitable aquaculture depends on good water quality. Physiologically, aquatic animals respond more intensely to their environment than do terrestrial animals. The stress of poor water can lead to disease and poor nutrition and retarded growth of cultivated aquatic animals. In Aquaculture the importance of Dissolved Oxygen is well known and it is one of the primary limiting factors of water quality parameters for success of aqua farming practices. The significance of Dissolved Oxygen to improve the ability of microorganisms to transform aquaculture pond contaminants to final products which are harmless to shrimp, fish, and environment has been a subject of laboratory investigations since the 90-ies. Now the oxidative microbiological treatment of contaminated soils and water is the technique of treatment of remediating contaminated aquaculture ponds. ZenLife is the combination of Oxygen releasing agent and multi strain probiotics formulation to address the water quality issues to enhance livability and sustainability by increasing Dissolved Oxygen levels in shrimp and fish aquaculture ponds.

Intensification of shrimp (P. vannamei, P. monodon) and fish (Carps, Pangassius, Tilapia, Seabass etc.) aquaculture practices resulted in a total departure from nature and resulted in increased disease incidence and other problems relating to productivity and environmental quality. Currently, our aim is to bring nature back into aquaculture systems by employing the principles of natural ecosystem to enhance productivity and make it a sustainable enterprise. Disease management, productivity and environment protection are the three key factors for successful and sustainable aquaculture.

In aquaculture systems, mostly Dissolved Oxygen is typically one of the major limiting factors and it causes sudden mortalities when the level of Dissolved Oxygen goes down beyond < 1 ppm. Dissolved Oxygen has essential role in all metabolic activities of shrimp including feed digestion, moulting and growth. At the same time Dissolved Oxygen limits the beneficial probiotics which are capable of aerobically biodegrading contaminants in the process of Bioremediation in aquaculture ponds. Without adequate Dissolved Oxygen, contaminated degradation will either cease or will proceed by a much slower anaerobic rate.

Oxygen Depletion in Aquaculture Ponds:
Oxygen depletion refers to low levels of Dissolved Oxygen and may result in shrimp or fish mortality. A concentration of >5 mg/L Dissolved Oxygen is recommended for optimum health and growth of shrimp and fish. Sensitivity to low levels of Dissolved Oxygen is species specific; however, most species of aquaculture are distressed when Dissolved Oxygen falls to 2-4 mg/L. Mortality usually occurs at concentrations less than 2 mg/L. The number of shrimp or fish that die during an oxygen depletion event is determined by how low the Dissolved Oxygen gets and how long it stays down.

Effect of Oxygen Depletion in ponds:
Oxygen depletion occurs when oxygen consumption exceeds oxygen production. Increases in oxygen consumption can be caused by an over-abundance of algae, "turnover" of a pond water (Thermal Stratification), increased organic waste entering the pond (i.e., waste feed or excess feed, faecal matter), death and decay of organic matter (i.e., algae, shrimp or fish die-offs), or by improperly dried pond having with sludge that remove oxygen directly from the water column.

Improved Dissolved Oxygen levels including Integrated Multi-trophic Aquaculture are now gaining interest for increasing in situ removal of organic waste in aquaculture systems. The use of a combination of Oxygen releaser and multi strain probiotics including nitrifying bacterial formulation will address the water quality issues by controlling the ammonia and organic sludge within the pond system. In view of this Neospark brings an innovative and unique product ZenLife for enhanced levels of Dissolved Oxygen and thereby improved Oxidative Bioremediation of organic waste of aquaculture ponds.

Oxidative Bioremediation is one of the Advanced and balanced Biotechnological tools and is the application of a biological treatment, mainly beneficial microbes, to clean-up hazardous contaminants in soil and surface or subsurface waters by the use of Oxygen in aquaculture ponds. In Oxidative Bioremediation microorganisms uses Dissolved Oxygen, which is readily available in ZenLife, to degrade organic contaminants in soil, sludge, water and solids. The beneficial probiotics of ZenLife feed on the contamination, deriving nutrition for growth and for reproduction with the help of Dissolved Oxygen. The beneficial probiotics of ZenLife will survive in the presence of proper dissolved oxygen levels and consume the waste organic matter source until the unwanted pollutant is gets remediated.

In general a successful bioremediation requires:
  • Availability of specific nutrients (N, P, K etc.) to support growth of these bacterial species
  • Abundance of electron acceptors (i.e. Dissolved Oxygen and secondary electron acceptors)
  • Contact with the dissolved and adsorbed contaminants
If any one of these requirements is not fulfilled, bioremediation will be inhibited, either partially or completely.

Oxidative bioremediation of ZenLife is more than just simply adding oxygen and a certain species of bacteria. Oxidative (Aerobic) bioremediation involves microbial reactions that require oxygen to go forward. The bacteria use a carbon substrate as the electron donor and oxygen as the electron acceptor. Whereas anaerobic bioremediation involves microbial reactions occurring in the absence of oxygen and encompasses many processes, including fermentation, methanogenesis, reductive dechlorination and sulfate- and nitrate reducing conditions. Depending on the contaminant, a subset of these activities may be cultivated. In anaerobic metabolism, nitrate, sulfate, carbon dioxide, oxidized materials or organic compounds may replace oxygen as the electron acceptor and thereby the end products are toxic to shrimp and fish (EPA 2006).

ZenLife is a unique formulas for the oxidative bioremediation of ponds, is standardized at a total count of 5 billion CFU /gm, which will multiply in a pond to 150 billion CFU/gm in 18 hours.

ZenLife is a state-of-the-art oxygen source for stimulating in situ oxidative bioremediation in aquaculture ponds. ZenLife enables aerobic beneficial probiotics to significantly accelerate rates of bioremediation over longer periods of time.

The oxygen and probiotics of ZenLife assist in degradation of excess food, feces, dead algae, reduction of turbidity thereby improved water and soil quality for better survival and growth of shrimp and fish in aquaculture ponds.

The use of ZenLife limits the presence of pathogenic microorganisms. ZenLife is ideal for use in all types of aquaculture ponds and garden aquaria to combat the most common problems associated with high nutrient levels.

ZenLife for ammonia reduction:
In aquaculture ponds, ZenLife is added life for ammonia reduction through nitrifying bacteria, as the ZenLife is designed to control ammonia and nitrite. Nitrification is the biological process in which ammonia is oxidized to nitrite by Nitrosomonas sp. and nitrite oxidized to nitrate by Nitrobacter sp.

  • Improves Dissolved Oxygen levels in pond water column.
  • Immediate relief to shrimp and fish during low Dissolved Oxygen levels.
  • Eliminates ammonia, nitrite and hydrogen sulfide through improved Oxidative Bioremediation.
  • Reduces BOD and COD levels and eliminates anaerobic condition.
  • Protects from stress and improves survival rates.
  • Controls stock losses due to low oxygen and high ammonia levels.
  • Reduce sludge buildup at pond bottom.
Shrimp and Fish Pond: 2-3 kg per acre or as advised by Aquaculture Consultant.

Application procedure: Broadcast uniformly throughout the pond.

Composition: Concentrated Oxygen releasing formulation in granular form containing Bacillus sp., Nitrosomonas sp., Nitrobacter sp., Rhodobacter sp., Rhodococcus sp.

Presentation: 5 kg

For Servicing Aquaculture Industry (Including Academic and Research Institutions, Universities etc.)
Alexander, M. 1999. Biodegradation and Bioremediation. Academic Press, San Diego USA. 453 pp.
Cao L et al, 2007. Environmental Impact of Aquaculture and Countermeasures to Aquaculture Pollution in China. Env Sci Pollut Res 14 (7) 452–462

Hanh D L, B.K. Rajbhandari & A.P. Annachhatre, 2005. Bioremediation of
Sediments from Intensive Aquaculture Shrimp Farms by Using Slow Oxygen Release
Agent. Journal of Environmental Technology Volume 26, Issue 5, 2005 pages 581-590

Pamela Chávez-Crooker and Johanna Obreque-Contreras, 2010. Bioremediation of aquaculture wastes. Journal of Current Opinion in Biotechnology, Volume 21, Issue 3, June 2010, Pages 313–317

Patricia Martínez Cruz et al, 2012. Use of Probiotics in Aquaculture. ISRN (International Scholarly Research Network) Microbiology, Volume 2012 (2012), Article ID 916845, 13 pages

Porubcan R. S., 1991. Reduction in chemical oxygen demand and improvement in Penaeus monodon yield in ponds inoculated with aerobic Bacillus bacteria, in Proceedings of the Program and Abstracts of the 22nd Annual Conference and Exposition, World Aquaculture Society, Puerto Rico, Spain, June 1991.

Shan, H. and Obbard, J. P. 2001. Ammonia removal from prawn aquaculture water using immobilized nitrifying bacteria. Appl. Microbiol. Biotechnol., 57: 791-798.

Sheryl O. Fernandes et al, 2010. Water quality and bacteriology in an aquaculture facility equipped with a new aeration system. Environmental Monitoring and Assessment, vol.164(4); 2010; 81-92

Shubhadeep Ghosh et al, 2010. Microcosm evaluation of indigenous microflora of traditional shrimp farming system as bioremediators. Indian J. Fish., 57(1) : 97-101

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